Egypt’s interior ministry released details on the attack, saying in an official statement that unknown assailants driving three 4x4 wheelers attacked by "randomly shooting" the bus carrying Copts, killing 26 and injuring 25.
The ministry added that an official count of the final death and injuries toll was underway.
A cordon was imposed around the site of the attacks, with the security forces intensifying efforts to catch the culprits, according to the statement.
The Copts were heading to Upper Egypt’s Minya Monastery of Anba Samuel the Confessor, located at western Adwa City.
Friday’s Minya attack is the latest in a series of deadly attacks on Egypt’s Christians, following the Palm Sunday Suicide Bombings last April.
Egypt has had an imposed nationwide three-month state of emergency after the April bombings, with the option to extend for another three months dependent on a parliament vote.