Khaled Mansour, a standup comedian on Bassem Youssef's El-Bernameg show, was questioned by prosecutors on Thursday morning.
Mansour, who is accused of insulting the president and insulting religion, was released on LE5,000 bail.
Bassem Youssef, Egypt's most prominent satirist, said on Wednesday via Twitter that Mansour would be questioned over a segment in the sixth episode of El-Bernameg that mocked President Morsi's allegedly ostentatious appearances at mosques for Friday prayers.
The Morsi government is "a failure and pathetic. It cannot handle a comedy show except through legal investigations," Youssef added in a separate tweet on Thursday.
In late March 2013, Youssef was interrogated by prosecutors for allegedly insulting the president and religion. He was released on bail of LE15,000.
Mansour is the second standup comedian to be questioned after appearing on El-Bernameg.
Ali Qandil was questioned by prosecutors in early April for allegedly insulting religion during an appearance on the show.