Strikers Manolo Gabbiadini and Federico Bernardeschi have been ruled out of Italy's internationals against Albania and the Netherlands due to injury, the Italian FA said on Monday.
Italy host Albania in a World Cup qualifier on Friday and travel to the Netherlands for a friendly four days later.
Gabbiadini, 25, who has been in fine form at Southampton, will not add to his six international caps after sustaining a groin injury in the English Premier League team's 2-1 defeat by Tottenham Hotspur on Sunday.
Bernardeschi, 23, who plays for Fiorentina in Serie A, did not play at the weekend due to "persistent ankle pain".
Italy coach Gian Piero Ventura has called up uncapped 21-year-old striker Andrea Petagna from Atalanta as a replacement.
Goalkeepers: Gianluigi Buffon (Juventus), Gianluigi Donnarumma (AC Milan), Alex Meret (Spal 2013)
Defenders: Davide Astori (Fiorentina), Andrea Barzagli (Juventus), Leonardo Bonucci (Juventus), Matteo Darmian (Manchester United), Danilo D’Ambrosio (Inter Milan), Mattia De Sciglio (AC Milan), Alessio Romagnoli (AC Milan), Daniele Rugani (Juventus), Leonardo Spinazzola (Atalanta), Davide Zappacosta (Torino)
Midfielders: Daniele De Rossi (AS Roma), Roberto Gagliardini (Inter Milan), Marco Parolo (Lazio), Marco Verratti (Paris Saint Germain)
Wingers: Federico Antonio Candreva (Inter Milan), Lorenzo Insigne (Napoli), Matteo Politano (Sassuolo), Nicola Sansone (Villarreal), Simone Verdi (Bologna)
Forwards: Andrea Belotti (Torino), Eder Citadin Martins (Inter Milan), Andrea Petagna (Atalanta), Ciro Immobile (Lazio)
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