Rosicky scored a goal Sunday to help Arsenal beat north London rival Tottenham 5-2, but was ruled out of Wednesday’s match in Dublin following a medical exam after the Premier League game.
The Czech football federation says coach Michal Bilek has called up Jiri Stajner of Czech club Liberec instead.
Bilek will also miss Hertha Berlin defender Roman Hubnik who has a foot injury. David Limbersky of Czech champion Viktoria Plzen was included instead.
The Czechs will play another warmup match against Israel on 26 May and take on Hungary on 1 June before opening its European Championship campaign against Russia on 8 June.
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