Italy and Manchester City forward Mario Balotelli
Balotelli scored in City's 3-0 win over Blackburn Rovers on Saturday, but he had previously been suspended for four matches after a stamp on Tottenham Hotspur's Scott Parker in January.
"I don't want players who commit reaction fouls and get sent off," Prandelli had already warned on Saturday.
"He seems a little agitated to me. When I say we have to reach the European Championship prepared, I mean I don't want to see players who, at the first sign of difficulty, commit reaction fouls and get sent off, leaving their team-mates to struggle with 10 men."
Balotelli has been replaced in the squad by Roma's 20-year-old striker Fabio Borini.
Italy's 23-man squad for Wednesday's friendly international against the United States in Genoa:
Goalkeepers: Gianluigi Buffon (Juventus), Salvatore Sirigu (Paris Saint-Germain/FRA), Morgan De Sanctis (Napoli), Edoardo Viviano (Palermo)
Defenders: Ignazio Abate (AC Milan), Federico Balzaretti (Palermo), Leonardo Bonucci, Giorgio Chiellini (both Juventus), Domenico Criscito (Zenit Saint-Petersburg/RUS), Christian Maggio (Napoli), Andrea Barzagli (Juventus), Angelo Ogbonna (Torino), Davide Astori (Cagliari)
Midfielders: Daniele De Rossi (Roma), Claudio Marchisio (Juventus), Riccardo Montolivo (Fiorentina), Thiago Motta (Paris Saint-Germain/FRA), Antonio Nocerino (AC Milan), Andrea Pirlo (Juventus)
Attackers: Sebastian Giovinco (Parma), Giampaolo Pazzini (Inter Milan), Alessandro Matri (Juventus), Fabio Borini (AS Roma).
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