Equatorial Guinea players celebrate victory over Tunisia in the 2023 Africa Cup f Nations qualifiers. Photo: CAF online
Striker Emilio Nsue scored the winning goal for the hosts five minutes before the final whistle from the penalty spot.
Equatorial Guinea now lead their qualifiers Group J with 12 points, while Tunisia, who had already qualified for the finals, came as the runner-up with 10 points.
With only one game remaining, Libya and Botswana are third and fourth in Group J with three and one point respectively.
In Group K, South Africa defeated Morocco 2-1, for the first time since 2002, but both teams have secured their place in the 2023 AFCON finals.
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