The Egyptian side opened their group stage campaign with a convincing 2-0 home victory over Mozambique's Black Bulls on 27 November, with midfield duo Ahmed Abdel-Rehim and Nabil "Dunga" Emad finding the net.
With three points from their opening fixture, the Cairo powerhouse share the top spot in Group D with compatriots Masry, who also won their first match.
The White Knights hold a solid track record against Nigerian sides, having won four of their six previous encounters, including two victories over Enyimba in the 2016 CAF Champions League group stage.
The Cairo giants will be eager to build on this history and solidify their position in the group.
Meanwhile, Enyimba seek to bounce back from a disappointing 2-0 defeat to Masry in their opening match.
The Nigerian side will aim to exploit home advantage and claim their first campaign points to remain in contention for progression.
Sunday's clash promises to be a pivotal contest in shaping Group D's early dynamics, with Zamalek striving to maintain dominance and Enyimba desperate for a turnaround.
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