Blasts were heard in Iran's capital, Tehran, an AFP journalist said, after a night of heavy bombardment mixed with thunder and rain.
It was not immediately clear what the targets were, but Israel's army said earlier it had launched a wave of strikes across Tehran, as well as strikes in Lebanon.
Lebanese state media reported Tuesday that Israeli strikes at dawn hit a residential building in Beirut's southern suburbs.
An AFP photographer saw firefighters tackling a blaze at the site of a strike, while rubble and debris were strewn across the road.
The war, now in its third week, has killed more than 1,200 Iranians and 850 Lebanese, and seen Iran launch retaliatory strikes against Israel and Gulf nations, as well as an Israeli front opening in Lebanon.
Air raid sirens sounded across Tel Aviv following warnings of incoming missiles launched from Iran, as a fresh wave of attacks targeted central Israel.
Israeli media reported that Iran had fired a cluster missile towards the country’s centre.
Iranian state television said a new wave of missiles had been launched less than an hour after an earlier barrage, suggesting a rapid succession of strikes.
Reports in Israeli media indicated that raids had hit at least three targets in central Israel, with accounts of shrapnel falling in affected areas.
Iraq has also been drawn in, with a drone and rocket attack targeting the US embassy in Baghdad on Tuesday, while a strike killed four people at a house reportedly hosting Iranian advisors, security officials said.
An AFP journalist reported seeing black smoke rising after an explosion in the embassy complex, as well as air defences intercepting another drone.
Millions of people have been displaced because of the war, notably in Lebanon and Iran, but the war has also hit the world economy as oil prices surge.
Oil prices rose more than five per cent on Tuesday after several countries pushed back on Trump's demand that they help protect shipping in the Strait of Hormuz, a waterway that is key to the transit of crude and liquefied natural gas.
The US and Israel struck Iranian oil infrastructure, and Iran has responded targeting the energy facilities of its crude-producing neighbours. At the same time, its threats against tankers transiting the Strait of Hormuz have all but closed the vital waterway.
A fifth of global crude oil passes through the Strait, and Trump has called on world powers, including US allies, to send warships to escort tankers -- so far in vain.
Armada to Hormuz
On Monday, Trump demanded that US allies join quickly and with "great enthusiasm" an armada to escort tankers through the strait.
He has warned that it would be "very bad" for the future of the NATO military alliance if the allies refused to help.
British Prime Minister Keir Starmer said London was working with allies to craft a "viable" plan to reopen the strait, but ruled out a NATO mission.
Berlin also said it "has been clear at all times that this war is not a matter for NATO".
Japan, Australia, Poland, Spain, Greece, and Sweden also distanced themselves from any military involvement in the Strait of Hormuz.
EU foreign ministers discussed the war in Brussels on Monday but showed "no appetite" for extending their Red Sea naval mission to help reopen the Strait of Hormuz, the bloc's top diplomat said.
Analysts said it was not surprising that America's partners were unenthusiastic about joining a war they had not been consulted on, after a year of tensions with Washington over everything from tariffs to Greenland.
The United States had "launched a war without consulting allies, expecting them to mop up the mess, and that's not going to fly" said Erwan Lagadec of George Washington University's Elliott School of International Affairs.
Defiant tone
Trump on Monday admitted he was "shocked" at Iran's response to the US-Israel attacks.
"They weren't supposed to go after all these other countries in the Middle East. Those missiles were set to go after them," he said.
"So, they hit Qatar, Saudi Arabia, UAE, Bahrain, Kuwait. Nobody expected that. We were shocked."
Iran's foreign minister struck a defiant tone on Monday.
"By now they have... understood what kind of nation they are dealing with," Abbas Araghchi told reporters in Tehran.
Iran, he said, "does not hesitate to defend itself and is ready to continue the war wherever it may lead, and take it as far as necessary".
Meanwhile, Western allies Canada, France, Germany, Italy, and the United Kingdom also urged Israel to show restraint in Lebanon, where it has announced ground operations.
Authorities in Lebanon have said more than one million people have registered as displaced since March 2, with more than 130,000 people staying in upwards of 600 collective shelters.
* This story was edited by Ahram Online.
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