The Serbian forward suffered a leg injury ahead of his team’s opening match against Real Madrid and has yet to appear in the tournament.
The Saudi club made history by reaching the quarter-finals after defeating English giants Manchester City 4-3 in the round of 16.
At this stage, Al-Hilal will face Brazilian side Fluminense, who eliminated European Champions League finalists Inter Milan.
Former Fulham striker Mitrović, who joined Al-Hilal in summer 2023, scored 28 goals in 36 appearances across all competitions last season.
Al-Hilal is also without talented winger Salem Al-Dawsari, ruled out for the rest of the tournament due to a muscle tear sustained during their group-stage match against RB Salzburg.
Find more details on group standings, match schedules, and competition statistics.
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