Yemeni police wounded several people when they opened fire on anti-regime demonstrators marching in the city of Taez on Tuesday, witnesses said.
Police fired "indiscriminantly" on protesters demanding the departure of President Ali Abdullah Saleh in the Wadi al-Qadi district of Taez, the second most-populated city of Yemen, some 200 kilometres (125 miles) south of Sanaa, according to witnesses.
They said several people received gunshot wounds, including a passerby who was seriously injured after being shot while in his car, they said.
Yemen's opposition remained adamant that veteran Saleh step down immediately, ahead of talks set for Tuesday between Gulf mediators and the government.
More than 125 people have been killed in the protests which broke out across the impoverished state late January.