Twitch, the gaming platform owned by Amazon, said Trump's channel was suspended over rules violations and that the offending content was removed.
"Hateful conduct is not allowed on Twitch," a spokesperson said.
"We do not make exceptions for political or newsworthy content, and will take action on content reported to us that violates our rules."
Twitch said the offending comments aired on the channel included Trump's remarks from 2015 saying that Mexicans coming into the United States were "bringing drugs," "bringing crime" and were "rapists."
Also cited were his statements from a recent Tulsa rally that "a very tough hombre is breaking into the window of a young woman whose husband is away."
Also Reddit on Monday said it yanked a forum used by supporters of President Donald Trump as part of a crackdown on hateful posts at the popular online bulletin board while the game streaming platform Twitch briefly suspended the president.
"r/The_Donald" was among some 2,000 forums or "subreddits" banned as tightening of rules at the news-focused social website, Reddit said.
The move comes with online platforms facing pressure to crack down on hateful content, including from the president, amid a broad movement to address racial equity in the wake of police killings of African-Americans.
Trump has complained that Silicon Valley platforms are biased against conservatives, despite his own large social media following.