The United States accused Russia of test-firing an anti-satellite weapon in space, warning that the threat against Washington's systems was "real, serious and increasing."
US Space Command said it "has evidence" that Moscow "conducted a non-destructive test of a space-based anti-satellite weapon" on July 15.
"Last week's test is another example that the threats to US and Allied space systems are real, serious and increasing," the Thursday statement continued.
"Clearly this is unacceptable," tweeted US nuclear disarmament negotiator Marshall Billingslea, adding that it would be a "major issue" discussed next week in Vienna, where he is in talks on a successor to the New START treaty.
The treaty caps the nuclear warheads of the US and Russia -- the two Cold War-era superpowers.
Commenting Friday on the accusations, Kremlin spokesman Dmitry Peskov said Russia supports "full demilitarisation of space and not basing any type of weapons in space."
The US Space Command said the test consisted of Russia's satellite called Cosmos 2543 injecting an object into orbit.
Russian state media has said that Cosmos-2543 had been deployed by another satellite, Cosmos-2542, which was launched on 25 of November 2019 by the Russian military.
The defense ministry said the satellite is meant to "monitor the condition of Russian satellites," but state daily Rossiyskaya Gazeta said it has the ability to "get information from somebody else's satellites."
The system is the same one that Space Command raised concerns about earlier this year, when it maneuvered near a US government satellite, said General Jay Raymond, head of US Space Command.
"This is further evidence of Russia's continuing efforts to develop and test space-based systems, and consistent with the Kremlin's published military doctrine to employ weapons that hold US and allied space assets at risk," Raymond said in a statement.
It is the latest example of Russian satellites behaving in a manner "inconsistent with their stated mission," the Space Command statement added.
"This event highlights Russia's hypocritical advocacy of outer space arms control," said Christopher Ford, a US assistant secretary of state for arms control.
The statement also came as China launched a rover to Mars on Thursday, a journey coinciding with a similar US mission as the powers take their rivalry into deep space.