Palestinian official news agency Wafa reported that the 15-year-old was left to bleed until he died by security forces at the checkpoint. Photo courtesy Wafa
The attack occurred at around 8:15 am (0615 GMT) at the Tunnels checkpoint south of Jerusalem when the teenager arrived on a bicycle, the force said in a statement.
A female soldier and a civilian armed guard were wounded in the stabbing, the police said, adding their injuries were "mild to moderate."
Israeli police later pronounced the Palestinian boy dead.
Palestinian official news agency Wafa reported that the 15-year-old was "left to bleed until he died" by security forces at the checkpoint.
The knife attack comes a day after a 12-year-old Palestinian boy died after being shot by Israeli border police at a refugee camp in Israeli-annexed east Jerusalem.
Rami Hamdan al-Halhuli, 12, was shot dead by Israeli forces while he was playing with his brother and friends in front of the family home in Shuafat refugee camp.
The police have not yet released Rami's body to the family, British broadcaster BBC reported.
The family told the BBC on Wednesday that the bullet hit Rami in his heart.
"There was no hope," his older brother Mahmoud, 19, who rushed to Rami the moment he was shot, told BBC. "He was already dead."
Rami was one of six Palestinians shot dead by Israeli forces in occupied East Jerusalem and the West Bank on Tuesday, BBC reported.
Hundreds of extra police have been deployed in the Old City of east Jerusalem since the Muslim fasting month of Ramadan began on Monday.
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