US Hailey Baptiste plays a forehand return to China's Xiyu Wang during their women's singles match on day 4 of the French Open tennis tournament at the Roland-Garros Complex in Paris. AFP
They withdrew on Thursday and no reason was given by organizers.
But Baptiste was forced to retire during her second-round singles against Wang Xiyu on Wednesday after landing awkwardly on her left leg late in the first set.
The Americans were replaced by Eudice Chong and Veronika Rejvec in the draw.
Williams, who turns 46 next month, was a singles wild-card entry at the Australian Open. She lost in the first round and became the oldest woman to compete in an Australian Open singles main draw.
A seven-time major singles winner, Williams previously held the No. 1 ranking in singles and doubles.
Williams lost the French Open singles final to younger sister Serena in 2002 and they twice won the French Open doubles together, in 1999 and 2010.
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